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. 2013 Feb 6;8(2):e55623. doi: 10.1371/journal.pone.0055623

Figure 7. Ezrin is necessary for cAMP mediated inhibition of NHE3, but not for the phosphorylation of NHE3 at serines 552 or 605.

Figure 7

C) Representative immunoblots and quantification (n = 3) of the phosphorylation of NHE3 at serine 605 A) and 552 B) of cells treated with forskolin/IBMX or vehicle, after transfection with either non-targeting siRNA or siRNA targeted against ezrin. D) NHE3 activity after treatment with forskolin/IBMX (pink squares and light blue crosses) or vehicle (yellow triangles or blue diamonds), after transfection with either non-targeting siRNA (blue diamond or pink square) or siRNA targeted against ezrin (yellow triangles or light blue crosses). E) Quantification of sodium dependent recovery of pH over the first 60 s of recovery after switching to sodium containing medium for the traces displayed in D. The data are represented as the mean ± SEM of at least 8 different experiments per condition, * represents a p value <0.05.