Table 2. Summary of S. flexneri strains expressing IcsA::BIO T330*G331* and IcsA::BIO G331W.

Plasmid	Codon#330–331	Amino acids	Polaritya	IcsA productionb	N-WASP recruitmentc	F-actin tailsd	Plaque formatione
pIcsA::BIO	ACT GGT	Thr Gly	P, P	+++	+++	+++	+++
pMYRM536	AGC GGG	Ser Gly	P, P	+++	+++	+++	+++
pMYRM537	GGG AGG	Gly Arg	P, Basic	+++	+/−	+/−	−
pMYRM538	GAG AAG	Glu Lys	Acidic, Basic	+++	++	++	++
pMYRM539	CCG GGC	Pro Gly	NP, P	+++	−	−	−
pMYRM543	GGG CCG	Gly Pro	P, NP	+++	−	−	−
pMYRM556	AAG AAC	Lys Asn	Basic, P	+++	++	++	++
pMYRM588 (pIcsA::BIO G331W)	ACT TGG	Thr Trp	P, NP	+++	++	++	++

^aP, polar; NP, non-polar.

^bThe “+++” symbol indicates relative band intensities of Western immunoblots of whole cell lysates.

^{c, d}“+++”, WT N-WASP recruitment/F-actin comet tail or capping formation; “++”, 20%–80% reduction in N-WASP recruitment/F-actin comet tail or capping formation; “+/−”, 90% reduction in N-WASP recruitment/F-actin comet tail or capping formation; “−”, N-WASP/F-actin tail not detected.

^e“+++”, WT plaques; “++”, small plaques; “−”, no plaques.