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. 2013 Feb 6;8(2):e55181. doi: 10.1371/journal.pone.0055181

Figure 5. UtMCs cultured in SMDM express cardiac lineage markers.

Figure 5

(A) RT-PCR analysis of cardiac genes expression in freshly isolated UtMCs (0 h) and UtMCs cultured for 3, 5, 10 and 15 days in SMDM. FH, are RNAs isolated from E17 mouse fetal hearts. (B) Immunofluorescence expression of Gata-4, Isl1, sACTN and cTnT in UtMCs cultured for 6 h and 5, 10 and 15 days in SMDM (C) Western blot analysis of Isl-1, GATA-4, sACTN and cTnT expression in freshly isolated UtMCs (0 h) and UtMCs cultured for 3, 5, 10 and 15 days in SMDM. FVO, are proteins extracted from E17 mouse fetal visceral organs (heart, lung, and peritoneal organs). (D) Double immunofluorescence detection of smoothelin-B with either sACTN or cTnT in UtMCs cultured 10 days in SMDM. (B, D) Nuclei are counterstained in blue with Hoechst 33342.