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. 2013 Feb 6;8(2):e55091. doi: 10.1371/journal.pone.0055091

Figure 4. Thalidomide lowers BACE1 and reduces β-site cleavage of APP.

Figure 4

Representative images of Western blots showed β-secretase enzyme BACE1 bands in WT and littermate APP23 mice with/without thalidomide administration (A) and a significant decrease in BACE1 amount was found with thalidomide application (A, Mean ± SD, *p<0.05, ANOVA test, n = 10 each group). BACE1 activity was normalized to the input protein amount and indicated as an arbitrary unit. The activity was measured and a lower activity of BACE1 was found with thalidomide application (B, Mean ± SD, *p<0.05, ANOVA test, n = 10 each group). Representative images of Western blots showed an amount decrease of sAPPβ secretion (C) and the density of bands significantly reduced in the thalidomide treated APP23 mice compared to littermate vehicle groups (C, Mean ± SD, *p<0.05, Students t-test, n = 10 each group). Microphotographic images of APP-CTF fragments showed an amount decrease of C99 following thalidomide administration (D). The density of bands was measured with a significant decrease in C99 fragments (E) but not significant changes of APP levels compared to vehicle groups (F) (Mean ± SD, **p<0.01, Students t-test, n = 10 each group).