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. Author manuscript; available in PMC: 2013 Aug 24.
Published in final edited form as: Chem Biol. 2012 Aug 24;19(8):1010–1019. doi: 10.1016/j.chembiol.2012.05.023

Figure 1. Strategy for ICC-guided peptidomics of individual cells.

Figure 1

The strategy has three experimental stages: (a) single-cell preparation (isolation and culturing), (b) immunolabeling, and (c) individual cell and tissue MS-based characterization, including peptide profiling and identification. Abundant peptides that were observed in the mass spectra are subsequently fragmented by tandem mass spectrometry to reveal the amino acid sequence. The three images in panel (b) show (1) a single RFamide ir PI cell with a cell size of 30 μm under ambient light condition, (2) the same cell as in (a) visualized with a fluorescence microscopy, and (3) merged image of (1) and (2). Scale bar = 50 μm.