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. Author manuscript; available in PMC: 2013 Feb 7.
Published in final edited form as: Curr Biol. 2004 Aug 10;14(15):1319–1329. doi: 10.1016/j.cub.2004.07.052

Figure 4. robo, robo2 and slit Mutations Cause Thoracic-to-Abdominal Transformations of Chordotonal Organs.

Figure 4

(A–D) Cho organs in stage 16 embryos visualized with mAb 22C10. (A) Wild-type thoracic dCh3 dorsally positioned and with dendrites pointing downward (green arrow), versus (B) wild-type lCh5 with dendrites pointing upward. (C) robo, robo2 double mutant; (D) slit mutant. Both have thoracic dCh3s positioned laterally with dendrites pointing upward (green arrow), adjacent to similarly oriented A1 lCh5 organs.

(E and F) Slit expression in a stage 13 wild-type embryo (E), showing strong expression in midline glia (mg) and in visceral mesoderm (vm); this expression is absent in a slit mutant embryo of the same age (F), imaged with identical settings. Slit is not detected in Cho organs.

(G and H) Expression of Robo and Robo2 proteins in wild-type stage 15 Cho neurons. Robo is expressed weakly in the cell bodies and strongly in the dendrite tips, while Robo2 is detected mainly in the dendrites (red arrows). Red star denotes muscle attachment site Robo expression. (G′) and (H′) show colocalization with elav::GFP (G′) or 22C10 (H′) to show location of Cho neurons.