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. Author manuscript; available in PMC: 2013 Nov 30.
Published in final edited form as: Oncogene. 2012 Jul 16;32(22):2756–2766. doi: 10.1038/onc.2012.294

Figure 2.

Figure 2

Effect of Jab1 depletion on the sensitivity of NPC cells to cisplatin, IR and UV irradiation. (a) Jab1 expression in NPC cells. CNE1, CNE2, and HONE1 cells in the logarithmic growth phase were collected and lysed, followed by western blotting for Jab1. β-actin was used as a loading control. (Right) Quantification of Jab1 expression. (b, c, d, e) Effect of knockdown or ectopic overexpression of Jab1 on the sensitivity of NPC cells to cisplatin, IR and UV. HONE1 (b) and CNE2 (c) cells were transiently transfected with a Jab1 siRNA (Jab1-si) or scrambled control siRNA (Cont-si), CNE2 (d) cells were stably transfected with Jab1 shRNA (sh-Jab1) or control shRNA (sh-Cont), CNE1 (e) cells were transfected with ectopic Jab1 (Myc-Jab1) or a control vector (pcDNA). Cells were then analyzed for their Jab1 expression level (left) and response to cisplatin (CP), IR irradiation (middle) UV (right) as described in the Materials and Methods. Data represent three independent experiments, mean±s.d. * P <0.05, **P <0.01.