Skip to main content
. Author manuscript; available in PMC: 2013 Nov 30.
Published in final edited form as: Oncogene. 2012 Jul 16;32(22):2756–2766. doi: 10.1038/onc.2012.294

Figure 3.

Figure 3

Effect of Jab1 on DNA damage-induced apoptosis. HONE1 cells were transiently transfected with either Jab1 siRNA (Jab1-si) or scrambled control siRNA (Cont-si) and treated with cisplatin (CP) (a) or IR (b) or UV (c) at the indicated doses. Apoptosis was analyzed by PI staining (left) or detection of cleaved PARP and caspase-3 by western blotting (right). (c) CNE1 cells were transfected with ectopic Myc-Jab1 plasmid and treated with cisplatin (CP) or UV for 48 h. Apoptosis was analyzed by Hoechst 33342 staining for condensed nuclei (left) and detection of cleaved PARP and caspase-3 by western blotting (right). Data represent three independent experiments, mean±s.d. *P <0.05, **P <0.01.