Figure 4.
Role of Jab1 in the response of NPC cells to cisplatin (CP), IR and UV. CNE1 and CNE2 cells were transiently transfected with Jab1 siRNA (Jab1-si) or scrambled control siRNA (Cont-si) and treated with CP, IR or UV for 48 h. Apoptosis was analyzed by Annexin-V and PI staining (a), detection of cleaved PARP (C-PARP) and caspase-3 (C-Caspase 3) (b, c and d). The percentage of CNE2 cells in the sub-G1 phase are shown (b and c, right). Data represent three independent experiments, mean±s.d. *P <0.05, **P <0.01.