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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Hepatology. 2013 Feb;57(2):577–589. doi: 10.1002/hep.26081

Figure 5. Palmitic acid activates inflammasome in cooperation with TLR2 ligand in Kupffer cells.

Figure 5

Kupffer cells were isolated from WT mice and cultured in the presence of 5 µg/ml Pam3CK4 and/or 200 µM palmitic acid. (A) mRNA expression of IL-1β and active IL-1β in the supernatant. (B) mRNA expression of IL-1α and active IL-1α in the supernatant. (C) mRNA expression in NLRP3. Genes were normalized to 18S RNA as an internal control. (D) Caspase-1 activity. (E) Immunobotting for the active form of caspase-1 is shown. N.D; not detected. Data represent mean ±SD, *p<0.05.