Skip to main content
. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Invest Dermatol. 2012 Oct 25;133(3):658–667. doi: 10.1038/jid.2012.364

Figure 1. Type I procollagen expression in aged human skin is restored by enhancing structural support of the dermal microenvironment.

Figure 1

Aged human skin was biopsied 4 and 12 weeks after injection of vehicle or filler. (A) Immunostaining of type I procollagen protein at 4 weeks (left panels) and 12 weeks (right panels) (n=21). Insets display elongated morphology of immunostained fibroblasts adjacent to pools of filler (†). Graphs display staining quantification. (B) The level of type I procollagen protein was determined by ELISA (n=22). Immunostaining localization of (C) prolyl-4-hydroxylase (n=22) and (D) heat shock protein 47 (HSP47, n=10) to fibroblasts adjacent to pools of filler (†) at 4 weeks (left panels) and 12 weeks (right panels), with quantification of staining. All images, bars=100μm; means+SEM. *p<0.05.