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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Hepatology. 2013 Feb 12;57(3):995–1004. doi: 10.1002/hep.26099

Fig. 7.

Fig. 7

Aged hepatocytes are selectively sensitized to death from the Fas death pathway. (A) Primary hepatocytes were isolated from young (2 month) and old (18 month) mice, cultured and the percentage cell death determined by MTT assay 24 h after treatment with the indicated μM concentrations of menadione (n=7–11). (B) Percentage cell death by MTT assay 24 h after treatment with TNF alone, or cotreatment with actinomycin D and TNF (Act/TNF) (n=4–8). (C) Percentage cell death after 24 h treatment with 0.25 or 0.5 mM palmitate (n=7–8). (D) Percentage cell death by MTT assay 24 h after treatment with the indicated amounts (ng/ml) of Jo2 antibody (*P<0.0001; n=4–8). (E) Percentage of acridine orange/ethidium bromide costained cells that were apoptotic or necrotic by immunofluorescence 12 h after treatment with 25 or 100 ng/ml of Jo2 antibody (*P<0.005, #P<0.03 as compared to young mice; n=3).