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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Microbiol. 2012 Oct 5;86(4):954–970. doi: 10.1111/mmi.12035

Fig. 8.

Fig. 8

Wrinkled colony formation by luxU and rscS mutants. Time-course assays of wrinkled colony formation induced by sypG overexpression using plasmid pEAH73. Cultures were spotted onto LBS medium containing Tet and incubated at 28°C. Wrinkled colony formation was monitored up to 43 h post-spotting for the following strains: ΔsypE control (pEAH73/KV3299) (A), ΔrscS ΔsypE (pEAH73/KV6268) (B), ΔluxU ΔsypE (pEAH73/KV4830) (C), and ΔrscS ΔluxU ΔsypE (pEAH73/KV6269) (D). An * indicates the time at which initiation of wrinkled colony formation was apparent, typically identified by the presence of ridges around the outer edge of the spot. Data are representative of at least three independent experiments.