Table 2.

Plasmids used in this study.

Plasmids
pEAH73	pKV69 carrying wild-type sypG; Cmr Tetr	(Hussa et al., 2008)
pEAH90	pEVS107 the PsypA promoter region (generated with primers 714 and 782) upstream of promoterless lacZ	This study
pEVS104	Conjugal helper plasmid (tra trb); Kanr	(Stabb & Ruby, 2002)
pEVS107	Mini-Tn7 delivery plasmid; oriR6K, mob; Kanr, Ermr	(McCann et al., 2003)
pJET1.2	Commercial cloning vector; Apr	Fermentas
pKV69	Mobilizable vector; Cmr Tetr	(Visick & Skoufos, 2001)
pKV276	pEAH73 with D53E mutation in sypG; Cmr Tetr	(Hussa et al., 2008)
pKV363	Mobilizable suicide vector; Cmr	(Shibata & Visick, 2012)
pSW7848	Mobilizable suicide vector; Cmr	Marie-Eve Val
pVAR17	pSW7848 containing 2 kb sequence flanking sypE derived from pCLD19	(Hussa et al., 2008) and this study
pVAR18	pSW7848 containing 3.3 kb sequence flanking luxU using primers 995, 996, 1017, and 1018	This study
pVAR29	pKV363 containing 850 bp sequencing flanking luxQ using primers 1286, 1287, 1288, 1304	This study
pVAR30	pKV363 containing 1.1 kb sequence flanking luxP using primers 1282, 1283, 1284, and 1303	This study
pVAR36	pKV363 containing 1.1 kb sequence flanking luxO using primers 1319, 1320, 1344, and 1345	This study
pVAR37	pKV363 containing 1.1 kb sequence flanking luxO and luxU using primers 1319, 1321, 1344, and 1346	This study
pVAR52	pEVS107 with PlacZ containing 2.3 kb luxQ-FLAG allele using primers 1314 and 1437	This study
pVAR53	pEVS107 with PlacZ containing 2.3 kb luxQ-A216P-FLAG allele using primers 849 and 1425	This study
pVAR54	pEVS107 with PlacZ containing 2.3 kb luxQ-H378A-FLAG allele using primers 849 and 1426	This study
pVAR55	pEVS107 with PlacZ containing 400 bp luxU-FLAG allele using primers 1312 and 1422	This study
pVAR56	pEVS107 with PlacZ containing 400 bp luxU-H61A-FLAG allele using primers 849 and 1427	This study
pVAR62	pKV363 containing 1.1 kb sequence flanking ainR using primers 1323 and pr_NL35 (Lyell et al., 2010)	This study