Table 1.
Size and lipid content of nanodiscs used in this study
| MSP protein | MSP: DMPC ratio | Size exclusion chromatography (Ve, MW, Rh×2)† | Diameter from EM (nm) | Rotational correlation time τc from NMR‡ (calc. MW) | DLS$ Rh×2 (nm), MW | Calc. diameter (nm)# |
|---|---|---|---|---|---|---|
| MSP1D1wt | 1:80 | 13.7 mL, 124kDa, 10.2nm | 9.5±1.1 | 48ns (158kDa) | 9.4±0.9 (126kDa) | 9.5 |
| ΔH4/2 | 1:60 | 14.10 mL, 102kDa, 9.4nm | n.d. | n.d. | n.d. | 8.9 |
| ΔH4 | 1:45 | 14.3 mL, 93kDa, 9.1nm | 7.8±0.8 | 33ns (102kDa) | 8.2±0.9 (91kDa) | 8.2 |
| ΔH5 | 1:50 | 14.25 mL, 95kDa, 9.2nm | 8.2±0.6 | 34ns (107kDa) | 8.4±0.7 (98kDa) | 8.4 |
| ΔH4/2H5 | 1:30 | 14.66 mL, 78 kDa, 8.4nm | n.d. | n.d. | n.d. | 7.9 |
| ΔH4H5 | 1:20 | 15 mL, 66 kDa, 7.8nm | 6.9±0.8 | 27ns (81kDa) | 7.3±0.3 (70kDa) | 7.1 |
| ΔH4-H6 | 1:~10 | 15.5 mL, 52kDa, 6.8nm | 6.3±0.6 (10.9±0.9) | n.d. | 6.4±0.5 (52kDa) | 6.3 |
†
Rh: hydrodynamics radius
‡
Obtained with [15N,1H]-TRACT experiments at 318K with 2H,15N labeled OmpX
$
DLS: dynamic light scattering
#
Calculated using the formula: D = (n(aa)·0.15nm/π) + 1nm, where n(aa) is the number of amino acids in contact with lipids, which is 180 for MSP1D1