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. 2013 Jan 15;27(2):163–168. doi: 10.1101/gad.207043.112

Figure 1.

Figure 1.

Identification of novel DAP proteins. (A) Schematic of SILAC experiment. DAP proteins belong to the group of proteins showing a 1:1 ratio. (B) Summary of the screen for DAP proteins. The SILAC ratio (H/L) is indicated. (C) RPE-1 cells in G1 were stained with antibodies to the indicated DAP proteins (red) or to centrin (green). Note the asymmetrical localization of DAP proteins. Bar, 1 μm. (D) Subcellular distribution of CEP83. RPE-1 cells stably expressing GFP-centrin 2 (green) were stained with an antibody to CEP83 (red) and DAPI (blue). Cell cycle stages are indicated; note that CEP83 is incorporated into centrioles during G2. (Bottom panel) In ciliated cells where primary cilia are marked with antibodies against acetylated α-tubulin (green), CEP83 localizes to the ciliary base. (E,F) RPE-1 cells stably expressing HA-tagged CEP83 were stained with antibodies to HA (red), Cep164 (green) (E) or Odf2 (green) (F) and centrin (blue) and examined with an OMX superresolution microscope (Applied Precision, Inc.). Note that CEP83 colocalizes with the DAP protein Cep164 both in top view (top panels) and side view (bottom panels) but does not colocalize with the SAP protein Odf2. Small bar, 200 nm.