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. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: Transfusion. 2012 May 25;53(2):382–393. doi: 10.1111/j.1537-2995.2012.03718.x

High titers of anti-A and anti-B antibodies influence platelet mediator release. Ratio of the mediator quantity at 24 hours to the original quantity in the sample is shown. The solid horizontal lines represent the mean of fold changes. Panel A: Fresh platelets from healthy blood group O (n=4), B (n=4) and A (n=15) individuals were exposed to a single group O plasma with an anti-A titer of 1:1024 and anti-B titer of 1:512. Supernatant thromboxane B₂ was measured after 24 hours of incubation at 37° C. The difference in thromboxane B₂ secretion between group A and non-A platelets was statistically significantly different (p = 0.04 by t-test with Welch’s correction). Panel B: The same supernatants shown in panel A were assayed for levels of sCD40L. Exposure of A platelets to a high titer anti-A antibody led to a significant decrease in supernatant sCD40L levels at 24 hours as compared with non-A platelets (p = 0.04).

High titers of anti-A and anti-B antibodies influence platelet mediator release. Ratio of the mediator quantity at 24 hours to the original quantity in the sample is shown. The solid horizontal lines represent the mean of fold changes. Panel A: Fresh platelets from healthy blood group O (n=4), B (n=4) and A (n=15) individuals were exposed to a single group O plasma with an anti-A titer of 1:1024 and anti-B titer of 1:512. Supernatant thromboxane B₂ was measured after 24 hours of incubation at 37° C. The difference in thromboxane B₂ secretion between group A and non-A platelets was statistically significantly different (p = 0.04 by t-test with Welch’s correction). Panel B: The same supernatants shown in panel A were assayed for levels of sCD40L. Exposure of A platelets to a high titer anti-A antibody led to a significant decrease in supernatant sCD40L levels at 24 hours as compared with non-A platelets (p = 0.04).