Fig. 1.

Ranking divalent cations as channel blockers in cell-based assays. a Efficacy of M²⁺ inhibitors of TRPV1 ranked by vanilloid-induced ⁴⁵Ca²⁺ uptake. b Co²⁺ inhibits heat-induced Ca²⁺ uptake at 37 °C and at 42 °C. c RuRed, R₄W₂ and AMI, previously validated selective channel blockers of TRPV1, were also tested for better comparison of inhibitors. Data are mean values ± standard deviation (SD) of the results of three independent experiments. Statistical significance was assessed by post hoc LSD t tests after significant one-way analysis of variance (ANOVA). P < 0.05