[A] Effect of AANAT knock-down on the mRNA expression of PCNA, SR, CFTR and Cl−/HCO3− AE2 in lysate from total liver samples and isolated cholangiocytes. There was increased mRNA expression of PCNA, SR, CFTR and Cl−/HCO3− AE2 in total liver and cholangiocytes from normal and BDL rats treated with AANAT Vivo-Morpholino compared to controls. Data are mean ± SEM of six real-time reactions performed in cumulative preparations (due to the low cell yield from 1 rat) of cholangiocytes obtained from 6 rats. *p<0.05 vs. the corresponding value of normal and BDL rats treated with mismatch Morpholino. #p<0.05 vs. the corresponding value of normal rats treated with mismatch Morpholino. [B] Effect of AANAT knock-down on the protein expression of PCNA, SR, CFTR and Cl−/HCO3− AE2 in lysate from isolated cholangiocytes. There was increased expression of PCNA, SR, CFTR and Cl−/HCO3− AE2 in cholangiocytes from normal and BDL rats treated with AANAT Vivo-Morpholino compared to controls. Data are mean ± SEM of 6 immunoblots performed in cumulative preparations of cholangiocytes obtained from 6 rats. *p<0.05 vs. the corresponding value of normal and BDL rats treated with mismatch Morpholino. #p<0.05 vs. the corresponding value of normal rats treated with mismatch Morpholino.