Table 1.
Evaluation of melatonin levels, the percentage of PCNA-positive or apoptotic cholangiocytes and intrahepatic bile duct mass and serum chemistry.
| Parameters | Normal rats + mismatch Morpholino | Normal rats + AANAT Vivo-Morpholino | BDL rats + mismatch Morpholino | BDL rats + AANAT Vivo-Morpholino |
|---|---|---|---|---|
| Serum melatonin levels (pg/ml) | 70.9 ± 1.1 (n = 5) | 77.6 ± 2.9a (n = 5) | 97.5 ± 2.8b (n = 5) | 174.1 ± 11.7c (n = 5) |
| Cholangiocyte melatonin levels (pg/ml) | 43.9 ± 4 (n = 4) | 26.9 ± 4.01a (n = 4) | 61.5 ± 3.5b (n = 4) | 39.5 ± 1.44c (n = 4) |
| % PCNA-positive cholangiocytes | 6.80 ± 2.13 | 10.24 ± 1.17a | 58.07 ± 7.25b | 68.10 ± 4.94c |
| IBDM (%) | 0.22 ± 0.1 | 0.41 ± 0.08a | 2.32 ± 0.41b | 4.75 ± 1.60c |
| % apoptotic cholangiocytes | Not detected | Not detected | 4.12 ± 2.30 | Not detected |
| SGPT (Units/L) | 62.6 ± 7.7 (n = 9) | 70 ± 1.25 (n = 9) | 194.4 ± 17 (n = 9) | 121.2 ± 19* (n = 9) |
| SGOT (Units/L) | 129 ± 7 (n = 9) | 148 ± 5.1 (n = 9) | 614.5 ± 68.3 (n = 9) | 312 ± 52* (n = 9) |
| ALP (Units/L) | 203 ± 6.6 (n = 9) | 202.8 ± 8 (n = 9) | 395 ± 9.8 (n = 9) | 343.2 ± 18.7* (n = 9) |
| Total bilirubin (mg/L) | < 0.1 (n = 9) | < 0.1 (n = 9) | 8.7 ± 0.5 (n = 9) | 7.5 ± 0.6* (n = 9) |
Data are mean ± SEM..
a
p<0.05 vs. the corresponding value of normal rats treated with mismatch Morpholino.
b
p<0.05 vs. the corresponding value of normal rats treated with mismatch Morpholino AANAT Vivo-Morpholino.
c
p<0.05 vs. all the other groups.
*
p<0.05 vs. the corresponding value of BDL rats treated with mismatch Morpholino. IBDM = Intrahepatic bile duct mass; area occupied by CK-19 positive-bile duct/total area x 100. SGOT = serum glutamic oxaloacetic transaminases; SGPT = serum glutamate pyruvate transaminases; ALP = alkaline phosphatase.