Fig. 5.
Prothrombin cleavage in EPA/DHA-treated platelets produces more alternative pathway by-products. Equal numbers of vehicle- or EPA/DHA-treated platelets were incubated with prothrombin, RGDS peptide, factor Xa, and 10 μg/ml collagen and lysed in reducing sample buffer at 15 s and 2, 3, 4, 5, 6, 7, 8, and 9 min. Lysates were blotted with an antibody recognizing prothrombin (PT, ∼80-kDa band), prothrombin-1 (PT-1, ∼55-kDa band), meizothrombin (MT, ∼43-kDa band; under reducing conditions, visible meizothrombin band does not have the B-chain), and fragment 2 (F2, ∼24-kDa band).