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Effect of copper loading on expression of copper-related genes. IEC-6 cells at 85% confluence were treated with 100–400 μM CuCl2 for 16 h. A–C: expression of copper transporter (Ctr1), SOD (Sod1), and antioxidant protein (Atox1) determined by quantitative RT-PCR. Values are means ± SD; n = 3.
