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Rate-equilibrium analysis of choline derivatives. On a log-log scale, the opening rate constant (f2) is plotted against the gating equilibrium constant (E2). The slope of the linear fit (Φ) was 0.90 ± 0.02. The agonists in this plot are (from top to bottom) ACh, TMA, BTMA, 4OH-BMTA, PTMA, ETMA, ClCho, 3OH-PTMA, cholamine pH 9.0, Cho, 2OH-PTMA, cholamine pH 6.1, betaine, and water (no added ligand).
