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Response of ao(HII) to variable concentrations of electrolyte. Samples contained lipids combined with different amounts (%, w/w) of proteins and suspended in different concentrations of NaCl buffered with 10 mM HEPES, pH 7.0, at 60°C. HSC samples contained 150 mM NaCl, 1.5 mM CaCl2, and 10 mM HEPES, pH 7.0. Closed symbols indicate measurements that detected only the hexagonal phase; open symbols indicate the presence of coexisting QII phases. (A) DOPE. (B) DOPE/DOPG (9:1, mol/mol).
