Table 1.

Insertion mechanism of the ALPS motif of ArfGAP1 in a DOPC/DOG (85:15) bilayer

MD run	MD length	Insertion sequence						Protein-bilayer LJ (kJ/mol)b
		Insertion 1			Insertion 2
		Time (ns)	Amino acid	Defect size before insertion (nm2)a	Time (ns)	Amino acid	Defect size before insertion (nm2)a
MD a	1 μs	59	F26	0.29	-	-	-	−295
MD b	1 μs	52	F26	0.75	437	F4	0.52	−591
MD c	400 ns	47	F26	0.23	218	F4	0.21	−552
MD d	400 ns	86	F26	0.21	-	-	-	−318
MD e	400 ns	103	L12	0.24	-	-	-	−442

^aThe defect size before insertion is the average value over the last nanosecond preceding insertion.

^bThe average protein-bilayer LJ energy is computed in the time window between 300 and 400 ns.