Table 2.
Insertion mechanism of the ALPS motif of ArfGAP1 and GMAP210 in different lipid bilayers
| Peptide | Lipid bilayer | MD run | MD length | Sequence of insertions (time)a | Insertions before 400 ns |
|---|---|---|---|---|---|
| ArfGAP1 | DOPC DOG | 1 | 1 μs | F26 (59 ns) | 7 |
| 2 | 1 μs | F26 (52 ns); F4(437 ns); L5 (440 ns); M9 (532 ns) | |||
| 3 | 400 ns | F26 (47 ns), F4 (218 ns); L5 (220 ns) | |||
| 4 | 400 ns | F26 (86 ns) | |||
| 5 | 400 ns | L12 (103 ns) | |||
| ArfGAP1 | DMPC | 1 | 400 ns | F19 (196 ns); W16 (360 ns) | 4 |
| 2 | 400 ns | - | |||
| 3 | 400 ns | - | |||
| 4 | 400 ns | F26 (133 ns) | |||
| 5 | 400 ns | F26 (218 ns) | |||
| GMAP210 | DOPC DOG | 1 | 1 μs | F33 (18 ns); M1 (28 ns); L5 (112 ns); I30 (155 ns); L26 (224 ns); W4 (310 ns); M37 (430 ns); L16 (431 ns); L38 (459 ns); L8 (533 ns) | 19 |
| 2 | 1 μs | M1 (24 ns); W5 (122 ns); L5 (294 ns); L23 (736 ns); L12 (810 ns); L16 (833 ns); L8 (848 ns) | |||
| 3 | 400 ns | L23 (19 ns); L26 (19 ns); I30 (20 ns) | |||
| 4 | 400 ns | L8 (52 ns); L23 (165 ns); L26 (245 ns) | |||
| 5 | 400 ns | W4 (49 ns); M1 (178 ns); L16 (329 ns); L26 (399 ns) | |||
| GMAP210 | DMPC | 1 | 400 ns | - | 8 |
| 2 | 400 ns | L12 (53 ns); L8 (191 ns); L5 (194 ns) | |||
| 3 | 400 ns | I30 (331 ns) | |||
| 4 | 400 ns | L26 (64 ns) | |||
| 5 | 400 ns | W4 (50 ns); L5 (50 ns); F33 (106 ns) |
a
Only the insertions of large hydrophobic residues (Phe, Trp, Met, Leu, and Ile) are considered.