Figure 4.

Atomic model of six domains of ACTN4 linked by a novel ternary complex between ABD, neck, and CaM2 resolved from known pairwise interactions. The full model (shown in the center) was built from homology models to published structures (PDB IDs of known pairwise interactions shown surrounding the central model). Monomer 1 containing ABD is colored green, except for CH1 (orange) and the neck region (magenta). Monomer 2 containing the CaM-like domain is colored light blue, with CaM1 (pink) positioned on top of the ABD-neck-CaM2 ternary complex. The black arrow points to the ABS on CH1, whose access to F-actin is partially blocked by the rod domains in the closed ABD structure. For clarity, the disordered N-terminal is not shown.