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. 2013 Feb 5;104(3):705–715. doi: 10.1016/j.bpj.2012.12.003

Figure 8.

Figure 8

Experimental validation of residue H189 that stabilizes the ternary complex. (A) ABD-CaM2 interface: H189 of ABD interacts with D874 of CaM2. The other two residues (K181 and K193) of the putative PiP-binding site on CH2 are also shown, with neither of them able to make contacts with CaM2. (B) Representative image (left) and quantitative results of three experiments (right) of Coomassie Blue G-250 stained polyacrylamide gels (as in Fig. 6B) for WT, H189L, and ΔPBD (see text) constructs of full-length human ACTN4.