(A) PWTs (g, mean ± SEM) in response to von Frey filament stimulation were monitored before and 7 days after nerve ligation. On day 7, HZ166 (16 mg / kg body weight) was injected i.p.. 45 min later flumazenil (Flu, ▼, ▽ 10 mg / kg body weight) or vehicle (Veh, ●, ○) were injected i.p. PWTs were monitored for 3 hours after injection. n = 6 mice per group. (B) Statistical analysis. AUC (g·h, mean ± SEM) was calculated for the time interval between administration of flumazenil or vehicle (45 min) and the end of the experiment (4 hours). **, P < 0.01 (unpaired t-test).