Fig. 7.
ConT-mediated changes depend upon the activation of low-density lipoprotein receptor-related protein 6 (LRP6) through agonists of the canonical Wnt-signaling pathway. Changes in p-LRP6, p-ERK1/2, and Cx43 after 30 min (A) or 4 h (C) of incubation with ConT that was generated from control MSCs or MSCs treated with IWP-2 (ConTIWP-2) are shown. Densitometric quantitation (B) of the blot shown in A revealed significantly reduced p-LRP-6 (n = 3), without significant reduction in Cx43 protein. At both time points p-ERK1/2 was increased well above control levels (n = 3). GAPDH was used as a loading control. The ConTIWP-2-treated HL-1 monolayer showed an attenuated increase in θ (D) compared with ConT-treated cultures (*P < 0.05 between treatment groups indicated). Rel, relative.