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. 2012 Dec 12;304(4):F440–F450. doi: 10.1152/ajprenal.00487.2011

Fig. 9.

Fig. 9.

Exaggerated inflammatory response in CHOP−/− glomeruli after LPS treatment. Glomeruli were isolated from CHOP−/− and WT mice and treated with or without LPS. RNA and protein were then collected from glomeruli. A: representative Western blot showing LPS induced increase in CHOP protein levels in WT glomeruli. B: qRT-PCR. CHOP mRNA expression was significantly increased in WT glomeruli after LPS treatment (*P < 0.05). C: qRT-PCR. LPS treatment induced an increase in expression of MIP2 in CHOP−/− but not in WT glomeruli. CHOP−/− glomeruli had 2.4-fold higher expression of MIP2 compared with WT glomeruli after LPS treatment (*P < 0.05). D: qRT-PCR. LPS treatment induced an increased expression of CXCL1 in both WT and CHOP−/− glomeruli. However, CHOP −/− glomeruli had 1.6-fold higher expression of CXCL1 compared with WT glomeruli (*P < 0.05). E: representative image of Western blot showing LPS-induced increase in phosphorylated IκB, p38, and JNK in glomeruli isolated from both WT and CHOP−/− mice. The increase in phosphorylated IκB and JNK was greater in CHOP−/− glomeruli than in WT glomeruli. Data are expressed as means ± SD.