Fig. 6.

Adaptive responses of proteins involved in renal ammonia metabolism in response to IC-Rhbg-KO in hypokalemic mice. This figure summarizes experiments performed to determine whether adaptive changes in the expression of other proteins involved in renal ammonia metabolism compensate partially for the lack of Rhbg expression in intercalated cells. We compared expression in hypokalemic mice with intact Rhbg expression to hypokalemic mice with IC-Rhbg-KO. A–D: PEPCK, PDG, glutamine synthetase, and Rhcg protein expression, respectively. In each panel immunoblots examining expression in the cortex and the outer medulla are shown along with graphic analysis of mean expression. There were no significant differences in the expression of any of these proteins, either in cortex or outer medulla, between hypokalemic mice with intact or intercalated cell-specific Rhbg deletion (N = 8 in each group).