Table 4.
mRNA concentrations of insulin-sensitive genes
| CON | AA | |
|---|---|---|
| Liver | ||
| IR-A | 1.00 ± 0.06 | 1.00 ± 0.07 |
| IR-B | 1.00 ± 0.07 | 1.14 ± 0.10 |
| PGC1A | 1.00 ± 0.17 | 1.23 ± 0.18 |
| PFK (liver isoform) | 1.00 ± 0.11 | 1.24 ± 0.21 |
| CYTOC | 1.00 ± 0.04 | 1.12 ± 0.07 |
| FAS | 1.00 ± 0.07 | 0.98 ± 0.10 |
| SREBP1C | 1.00 ± 0.13 | 1.80 ± 0.51 |
| Skeletal muscle | ||
| IR-A | 1.00 ± 0.08 | 0.90 ± 0.10 |
| IR-B | 1.00 ± 0.07 | 0.87 ± 0.12 |
| PGC1A | 1.00 ± 0.11 | 0.96 ± 0.10 |
| PFK (muscle isoform) | 1.00 ± 0.08 | 0.95 ± 0.09 |
| CYTOC | 1.00 ± 0.08 | 1.30 ± 0.11∗ |
| NRF1 | 1.00 ± 0.09 | 0.86 ± 0.10 |
| NRF2 | 1.00 ± 0.04 | 1.10 ± 0.09 |
| ERRA | 1.00 ± 0.09 | 1.11 ± 0.14 |
Values are means ± SE; CON (n = 8) and AA (n = 7). IR-A, insulin receptor isoform A; IR-B, insulin receptor isolform B; PGC1A, PPARγ coactivator-1α; PFK, phosphofructokinase; CYTOC, cytochrome c; FAS, fatty acid synthase; SREBP1C, sterol response element-binding protein-1; NRF1, nuclear regulatory factor 1; NRF2, nuclear regulatory factor 2; ERRA, estrogen-related receptor-α. mRNA concentrations are relative to the average of 3 reference genes for skeletal muscle samples (β-actin, GAPDH, and S15) and 2 reference genes for liver samples (S15 and 18S), which were not different between groups. Results are presented relative to expression in the CON group.
∗
P < 0.05 between CON and AA.