Table 2.

RT-qPCR primer sequences

	Sequence (5′-3′)
RT-qPCR Primers	Forward	Reverse	Product Size	Dissociation Temperature, °C
Pck1	GGAAGGACAAAGATGGCAAGTTC	AGGCGTTTTCCTTAGGGATGTAG	138	87.3
Hmgcs2	TGGTTCAAGACAGGGACACAGAAC	AGAGGAATACCAGGGCCCAACAAT	98	84.1
Bdh1	TGCAACAGTGAAGAGGTGGAGAAG	CAAACGTTGAGATGCCTGCGTTGT	109	85
Rpl32	CCTCTGGTGAAGCCCAAGATC	TCTGGGTTTCCGCCAGTTT	102	81.8

Pck1, phosphoenolpyruvate carboxykinase 1; Hmgcs2, hydroxymethylglutaryl-CoA synthase 2; Bdh1, d-β-hydroxybutyrate dehydrogenase.