Table 4.

FSR of isotope-enriched fatty acids in HepG2 cells cultured with different PL concentrations

	PL, nM
FSR	10	20	50	2,000
[U-13C]18:3n-6	0.084 ± 0.006	0.091 ± 0.011	0.065 ± 0.030	0.093 ± 0.005
[U-13C]20:3n-6	0.048 ± 0.004	0.052 ± 0.004	0.054 ± 0.008	0.056 ± 0.004
[U-13C]20:4n-6	0.017 ± 0.001a	0.019 ± 0.001a	0.018 ± 0.001a	0.022 ± 0.001b**
[D33]C18:1n-9	0.043 ± 0.002a	0.046 ± 0.001a	0.047 ± 0.002a	0.051 ± 0.001b*
[D5]C18:4n-3	0.104 ± 0.004	0.106 ± 0.006	0.110 ± 0.004	0.109 ± 0.010
[D5]C20:5n-3	0.069 ± 0.002a	0.070 ± 0.002a	0.078 ± 0.004b	0.078 ± 0.005b
[D5]C22:6n-3	0.015 ± 0.001	0.016 ± 0.001	0.015 ± 0.001	0.016 ± 0.001

Data were presented as means ± SD; n = 3 experiments. Units are h⁻¹. FSR, fractional synthesis rate. Means of each row without a common letter in the superscript differ significantly (*P < 0.01 and **P <0.001).