Table A1.

Primer sequences of genes of interest in unsaturated fatty acid synthesis (Homo sapiens) retrieved from GenBank

Genes of Interest	Primer Sequences
FADS1 (F)	5′-TGATGTCTGGGTCTTTGCGGA-3′
FADS1 (R)	5′-TATGCCGTACAACCACCAGCAC-3′
FADS2 (F)	5′-AACATGATTATGGCCACCTGTCTGT-3′
FADS2 (R)	5′-TGGAAGATGTTAGGCTTGGCGT-3′
SCD (F)	5′-TCTGGAGAAACATCATCCTTATGTCTCT-3′
SCD (R)	5′-ACAGACGATGAGCTCCTGCTGTTAT-3′
ELOVL2 (F)	5′-TCTGCTCTCAATATGGCTGGGTAA-3′
ELOVL2 (R)	5′-TGCGCTGGTAAGATCTTGACACTG-3′
ELOVL5 (F)	5′-ATTCTCTTGCCGGGGGATTTTA-3′
ELOVL5 (R)	5′-TGCGTGTGCCCTGACAGAAG-3′
GAPDH (F)	5′-CTGCACCACCAACTGCTTAG-3′
GAPDH (R)	5′-AGGGAGGGGAGCCGGCTGTC-3′

F, forward primer; R, reverse primer; FADS1, Δ-5 desaturase; SCD, Δ-9 desaturase; FADS2, Δ-6 desaturase; ELOVL, elongase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.