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. 2012 Dec 13;304(3):G241–G256. doi: 10.1152/ajpgi.00334.2012

Fig. 2.

Fig. 2.

Effect of PUFA and high glucose on PPARα transcription of proteins in LCFA uptake/transport [liver fatty acid binding protein (L-FABP), acyl-CoA binding protein (ACBP)] and nuclear regulation (PPARα, HNF4α, HNF1α) in WT mouse hepatocytes. Total RNA was isolated from WT mouse hepatocytes treated with Alb only (40 μM) or PUFA (AA, EPA, or DHA, 200 μM) and Alb with 6 or 20 mM glucose for 6 h. The RNA was analyzed by quantitative PCR for changes in L-FABP mRNA (A), ACBP mRNA (B), PPARα mRNA (C), HNF4α mRNA (D), and HNF1α mRNA (E). Values are means ± SE, n = 4, P < 0.05. *Significant difference between lipid and Alb at constant concentration of glucose; #significant difference between 6 and 20 mM glucose for the same lipid.