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. 2012 Dec 13;304(3):G241–G256. doi: 10.1152/ajpgi.00334.2012

Fig. 3.

Fig. 3.

L-FABP gene ablation markedly reduced high glucose potentiation of PUFA-mediated PPARα transcription of genes in LCFA β-oxidation, transport (ACBP), and PPARα. As indicated by the vertical bars from left to right, L-FABP-null hepatocytes were cultured for 6 h in serum-free medium containing glucose (6, 11, 20 or 30 mM) supplemented with either fatty acid-free Alb (40 μM) or Alb complexed with 200 μM AA, EPA, or DHA as described in materials and methods. Total RNA was isolated and relative fold change in the expression level of PPARα-regulated genes was determined by quantitative PCR: CPT1A mRNA (A), CPT2 mRNA (B), ACOX1 mRNA (C), ACBP mRNA (D), and PPARα mRNA (E). Values for each genotype were expressed relative to Alb + 6 mM glucose within that genotype. Values are means ± SE, n = 3–4. *P < 0.05 vs. Alb alone at the same glucose concentration; #P < 0.05 vs. 6 mM glucose concentration within each treatment group.