Table 1.

Impact of high glucose on ability of n-3 PUFA to induce LCFA β-oxidation in cultured primary hepatocytes

Preincubation (24 h)		Postincubation (24 h)
BSA/Complex	Glucose, mM	BSA/Complex	Glucose	Stearic Acid Oxidation, nmol/mg protein
BSA	6	BSA/stearic acid	6	680 ± 13
BSA/n-3 EPA	6	BSA/stearic acid	6	744 ± 7*
BSA	20	BSA/stearic acid	20	1,015 ± 9†
BSA/n-3 EPA	20	BSA/stearic acid	20	1,299 ± 11*‡

Primary hepatocytes from wild-type mice were incubated for 24 h with 6 or 20 mM glucose in medium containing either BSA or BSA/200 μM n-3 eicosapentaenoic acid (EPA) as described in legend to Fig. 8. To determine the ability these hepatocytes to β-oxidize stearic acid, the hepatocytes were then further incubated for 24 h with 6 or 20 mM glucose in medium containing BSA/200 μM stearic acid plus trace quantity of [³H]stearic acid as inmaterials and methods. PUFA, polyunsaturated fatty acids; LCFA, long-chain fatty acid. Values are means ± SE, n = 6.

^*P < 0.05 vs. BSA at same glucose concentration;

^†P < 0.05 vs. BSA at 6 mM glucose.

^‡P < 0.05 vs. BSA/EPA at 6 mM glucose.