Fig. 1.
Sodium/hydrogen exchanger (NHE) 8 expression in the stomach in mice. A: RNA was isolated from the nonglandular and glandular regions of the stomach and used for PCR analysis. NHE8 mRNA and TATA-binding protein (TBP) mRNA were amplified with mouse-specific primers. The changes in NHE8 gene expression were analyzed by the comparative cycle threshold (Ct) method. Data are means ± SE from a total of 10 mice. *P ≤ 0.0002 for nonglandular region vs. glandular region (fundic glands region and pyloric glands region) of the stomach; #P ≤ 0.02 for fundic glands region vs. pyloric glands region of the stomach. B: total tissue lysate was prepared from the nonglandular and glandular regions of the stomach and used for Western blot detection. Protein (30 μg) was separated on 8% SDS-PAGE and immunoblotted with NHE8 antibody (1:3,000 dilution) and GADPH antibody (1:10,000 dilution). C: stomach tissue sections were prepared from mice and reacted with NHE8 antiserum at 1:200 dilution. Immunohistochemical staining results were analyzed by MRC-1024ES laser-scanning confocal microscopy. D: stomach tissue sections were prepared from mice, and periodic acid-Schiff (PAS) staining was performed on tissue sections. Results were observed under a Zeiss Anxioplan microscope.