Fig. 2.

Average number of AQP1-positive DTLs, AQP1-negative DTLs, and ascending thin limbs (ATLs) grouped according to their nearest-neighbor CD distance (means ± SE, n = 4 kidneys, from 4 animals). The numbers of segments were grouped into bins that each span 3 μm. Nearest-neighbor CD distances for 200 of each segment, from each kidney, were measured on a transverse tissue section located ∼1,000 μm below the outer medullary-inner medullary (OM-IM) boundary. The number of ATLs per bin declines exponentially with respect to nearest CD distance (y = 73.3e^−1E−03x; R² = 0.94). See results for additional details.