Table 3.
Profibrotic gene expression in young mice
| Saline Treated | Hgb Treated | Fold Increase | P Value | |
|---|---|---|---|---|
| TGF-β1 | 1.7 ± 0.3 | 3.4 ± 0.4 | 2.0 | 0.017 |
| Smad3 | 3.2 ± 0.3 | 6.8 ± 0.4 | 2.1 | 0.0001 |
| Col I | 2.7 ± 1.0 | 9.5 ± 0.7 | 3.5 | 0.0004 |
| Col III | 4.7 ± 1.7 | 15.9 ± 1.1 | 3.4 | 0.0003 |
| Col IV | 2.8 ± 0.5 | 5.6 ± 0.4 | 2.0 | 0.002 |
Data are expressed as means ± SE. mRNA expression was assessed by quantitative real-time RT-PCR, and values are the result of relative quantification performed against a standard curve constructed for each mRNA target, normalized for expression of 18S rRNA, and expressed in arbitrary units; n = 4 and n = 6 for saline-treated and Hgb-treated mice, respectively. TGF-β1, transforming growth factor β1; Col I, III, and IV are collagen I, III, and IV, respectively.