Table 1.

mRNA of splenocytes reacted with the antigenic HSP70 peptide

mRNA	WKY (n = 5)	Tolerized (n = 5)	SSHTN (n = 5)	P
IL-2	0.65 ± 0.19	0.74 ± 0.29	4.33 ± 2.66	ns
IL-6	1.00 ± 0.25	0.71 ± 0.42	2.30 ± 0.26†	0.010
IL-10	0.69 ± 0.30	2.79 ± 0.87†	0.05 ± 0.05	0.008
IL-17	1.07 ± 0.28	1.76 ± 0.81	1.39 ± 0.16	ns
IDO	0.72 ± 0.38	0.59 ± 0.25	0.91 ± 0.25	ns
FoxP3	1.00 ± 0.32	1.28 ± 0.15	0.81 ± 0.19	ns
CTLA-4	1.00 ± 0.01	3.78 ± 1.21‡	1.70 ± 0.16	0.041
TGF-β	1.00 ± 0.27	2.97 ± 0.49	1.33 ± 1.61	ns
TGF-α	0.99 ± 0.02	0.95 ± 0.34	1.85 ± 0.44	ns

Data are means ± SE. RNA of splenocytes from rats of the salt-sensitive hypertension (SSHTN) group, tolerized group, and control [Wistar-Kyoto (WKY)] group incubated for 24 h with the antigenic HSP70 peptide. Data represent relative mRNA (quantitative PCR) in relation to the WKY values. P values listed are those considered significant (P < 0.05).

^†Significant differences from the rest (higher for IL-6 in SSHTN group and higher IL-10 in the tolerized group.

^‡Significant differences between tolerized and WKY. ns = P > 0.05.