Fig. 3.

Variations in the cystic phenotype produced by STAT5a/b siRNAs in nucleated HPAECs. A: HPAECs were transfected with the respective siRNAs as indicated together with an expression vector for myc-CLIMP63. Cellular phenotype was evaluated using an antibody for endogenous RTN4 and an anti-myc mAb to label the exogenously expressed myc-CLIMP63. Scr, scrambled. Scale bar = 10 μm. B: variation in endogenous RTN4 and CLIMP63 immunofluorescence in two cystic cells side-by-side in the same culture transfected with STAT5a/b siRNAs. Scale bar = 10 μm. C: variations in cell size of nucleated or enucleated cells with or without the cystic phenotype as measured using phase-contrast microscopy and ImageJ (7, 8). n = no. of cells sized in each group; N, nucleated cells; E, enucleated cells. *P < 0.05.