Fig. 4.

Addition of CLIMP63 siRNA enhanced development of cystic ER phenotype in response to STAT5a/b siRNAs. A: HPAEC cultures were transfected with respective siRNAs as indicated, and development of the cystic ER phenotype was evaluated 18 h later by phase microscopy. Scale bar = 25 μm. B: quantitation of % cystic cells in the respective cultures in A. n = no. of cells enumerated; error bars indicate means ± SE derived from replicate cultures (at least in triplicate). *,#P < 0.05 using Student's t-test. C: Western blot analyses showing knockdown of respective proteins by the indicated siRNAs. HPAEC cultures treated with respective siRNAs as in A for 18 h were harvested (9) and the cell extracts were evaluated for respective proteins by Western blotting of equal aliquots of total protein derived from each experimental group.