Fig. 5.

Development of cystic ER phenotype is unaffected by 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) but is inhibited by cycloheximide (CHX). A: HPAEC cultures were transfected with the respective siRNAs in the presence of DRB (75 μM) beginning 5 h before the transfection and the inhibitor was maintained in the cultures overnight. Development of the cystic ER phenotype was assessed by phase-contrast microscopy and quantitated as in Fig. 4B (means ± SE; NS = not significant; P > 0.05 using Student's t-test). Arrows highlight cystic cells. Scale bar = 25 μm. B: HPAEC cultures were transfected with the respective siRNAs in the presence of CHX (5 μg/ml) beginning 5 h before the transfection and the inhibitor was maintained in the cultures overnight. Development of the cystic ER phenotype was assessed by phase-contrast microscopy and quantitated as in Fig. 4B (means ± SE; *P < 0.05 using Student's t-test). Arrows highlight cystic cells. Scale bar = 25 μm.