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. 2012 Nov 21;304(4):C299–C311. doi: 10.1152/ajpcell.00302.2012

Fig. 3.

Fig. 3.

BK expression in Tg-BKR207Q SCNs. A-B: Tg-BKR207Q day (A) and night (B) SCN sections incubated with an α-BK antibody (red) and α-NF200 (green). BK is expressed throughout the SCN at both times. C: representative α-BK and α-tubulin (DM1α) Western blots from individual WT and homozygous Tg-BKR207Q SCNs harvested at 6-h time points. D: α-BK expression at each time point as a proportion of ZT20. WT BK expression over time (n = 4 SCNs at each time point) was different from Tg-BKR207Q (n = 3), P = 10−4, interaction effect with two-way ANOVA [≠ZT2, P = 0.02; ZT2 (cycle 2), P = 0.004, Bonferroni post hoc]. WT SCNs had a robust circadian difference in expression (P = 10−6, one-way ANOVA; Bonferroni post hoc between ZT2 and ZT20, P = 10−6), but the difference was less robust for Tg-BKR207Q across time points (P = 0.04, one-way ANOVA; Bonferroni post hoc between ZT2 and the ZT14, P = 0.11). E: real-time RT-PCR expression of Per2 and Bmal1 in WT and Tg-BKR207Q SCNs at ZT6 (day) and ZT19 (night), normalized to α-tubulin. Per2 and Bmal1 expression was both significantly different between day and night (*P = 0.02 and 0.01, respectively, time effect with two-way ANOVA) but not between genotypes (P = 0.29 and 0.42, genotype effect with two-way ANOVA). WT: n = 3 (day), 3 (night); Tg-BKR207Q: n = 2, 3.