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. 2013 Feb 7;33(2):e00023. doi: 10.1042/BSR20120104

Figure 7. Cellular distribution of Drp1/Fis1 and colocalization of these proteins as visualized by immunofluorescence confocal microscopy.

Figure 7

(A) Representative micrographs of Drp1 and/or Fis1 immunostaining taken with a confocal microscope. LINCL cells were cultured on coverslips and were incubated with (LINCL+TPP-1) or without (LINCL) TPP-1 before being fixed, permeabilized and incubated with antibodies raised against Fis1-(red) or Drp1-(green). In merged micrographs, the appearance of the yellow fluorescence signals reflects the colocalization between both proteins. Nuclei were stained with TO-PRO-3 iodide (blue fluorescence). (B) The co-localization between Drp1 and Fis1 was quantified using the co-localization module of Leica LAS AF software. The results are expressed as the percentages of co-localization (means±S.D.; n=20). NS, non-significantly different from LINCL cells, as determined by a Student's t test.