Fig. 2.

Location of cells and kinetics of Mtb growth after Ad85A immunization. (A) BALB/c mice in IVCs were immunized with Ad85A i.d. or i.n. Four weeks post immunization BAL was collected from individual mice and the number of CD8+, CD8IFNγ+ and CD8CXCR6+ cells determined by flow cytometry. The figures show the mean number of each cell population recovered ±SD from 3 mice in one of three independent experiments ***p ≤ 0.005. (B) Kinetics of Mtb growth after Ad85A i.d. and i.n. immunization. BALB/C mice were immunized in IVCs once with Ad85A i.d. or i.n. Five weeks after immunization mice were challenged with Mtb and groups of 5 mice sacrificed 7, 14, 21 and 28 days later for enumeration of lung Mtb CFU. ***p < 0.001, *p < 0.05 compared to naïve animals, one-way ANOVA with Tukey's post test. Representative data from one of two experiments are shown.