Fig. 4.

Mtb CFU after CD25 depletion. Unimmunized (N) or Ad85A i.d. immunized BALB/c mice in IVCs were given drinking water with or without M. abscessus (M abs). Five weeks after the immunization they were challenged with Mtb i.n. and after further 5 weeks sacrificed for enumeration of lung and spleen Mtb CFU. The day before and 7 and 14 after the Mtb challenge, mice were given CD25 antibody or control rat Ig i.p. Representative data from one of two experiments with 5–7 mice/group are shown. ***p < 0.001, **p < 0.01, *p < 0.05 compared to unimmunized animals with clean water, one-way ANOVA with Tukey's post test.